Figure 1
From: Cheminformatics methods for novel nanopore analysis of HIV DNA termini
A nanopore device based on the α-hemolysin channel (from [41]). a) Diagram of a horizontal bilayer apparatus used in the UNO-RIC laboratory. One α-hemolysin channel is intercalated in a horizontal bilayer. The bilayer is supported on a 25-micron-diameter conical aperture at the end of a U-shaped Teflon tube. The tube connects two 70 μl volume baths filled with 1 M KCl buffered at pH 8.0. b) Two-dimensional diagram of a 9 bp hairpin captured in the pore vestibule. The stick figure in blue is a two dimensional section of the α-hemolysin pore derived from X-ray crystallographic data. c) Representative blockade of ionic current caused by a 9 bp DNA hairpin (9 bpC•G). Open channel current (Io) is typically 120 pA at 120 mV and 23.0°C. In the case of 9 bp hairpins, the residual current transitions between four levels.