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Table 3 Comparison of annotations of PPIs common to literature-derived curated PPI databases

From: An evaluation of human protein-protein interaction data in the public domain

  Interacting Proteins HPRD DIP BIND MIPS MINT IntAct
    Detection method PubMed ID Detection method PubMed ID Detection method PubMed ID Detection method PubMed ID Detection method PubMed ID Detection method PubMed ID
1 NFKB1 NFKB3 in vivo 9101089 Tandem Affinity Purification (TAP) 14743216 Gel retardation assays, three dimensional structure 15735750, 9738011, 9865693 - - anti tag coimmuno- precipitation 14743216 Comigration in gel, anti bait coip, tap 8246997, 8246997, 14743216
2 TNFRSF1A TRADD in vivo, in vitro, Yeast 2-hybrid 7758105, 8565075, 8612133 Experimental 9129204 Immuno- precipitation 11684708, 15247912, 9916731 coip: coimmuno precipitation 9916731 Coimmuno- precipitation, pull down, two hybrid 8565075, 8621670 anti bait coip, pull down, two hybrid 7758105
3 FADD FAS in vivo, in vitro, Yeast 2-hybrid 8967952, 7538907, 7536190 Two hybrid test 7538907 Immuno- precipitation 15665818, 15383280 coip: coimmuno precipitation 10196099 Coimmuno-precipitation, two hybrid 7536190, 7538907 anti tag coip, coip, pull down, two hybrid 7538907, 7536190, 7538907, 7538907
4 PEX19 PEX3 in vivo, in vitro, Yeast 2-hybrid 10704444, 12096124 - - two-hybrid-test 10430017, 12096124 coip: coimmuno precipitation, two hybrid 10430017 two hybrid, ubiquitin reconstruction 12096124, 16189514 far western blotting, two hybrid pooling 10704444, 16189514
5 CDK2 CDKN1A in vitro 12839982 Two hybrid test 8242751 other 15232106 coip: coimmuno precipitation 8641969 protein array, pull down 15232106, 9284049 protein array, pull down 15232106, 8756624
6 PEX12 PEX5 in vivo, in vitro, Yeast 2-hybrid 10562279, 10837480, 12096124 - - two-hybrid-test 12096124 coip: coimmuno precipitation, two hybrid 10646847 two hybrid 12096124 anti tag coip, filter binding, two hybrid 10562279, 10562279, 12620231