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Figure 3 | BMC Bioinformatics

Figure 3

From: Use of DNA melting simulation software for in silico diagnostic assay design: targeting regions with complex melting curves and confirmation by real-time PCR using intercalating dyes

Figure 3

Comparison of melting profiles obtained using different intercalating dyes and purified Naegleria amplicons. First derivative plots for purified amplified DNA from Naegleria fowleri (red line), Naegleria lovaniensis (blue line) and Naegleria australiensis (black line) melted in the presence of either SYTO9 (A), SYBR Green (B), LC Green I (C) or SYTO59 (D).

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