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Figure 4 | BMC Bioinformatics

Figure 4

From: Use of DNA melting simulation software for in silico diagnostic assay design: targeting regions with complex melting curves and confirmation by real-time PCR using intercalating dyes

Figure 4

Comparison of real-time PCR and simulated melting profiles for different cyanobacterial species. Plots (solid lines) of the first derivatives (for either fluorescence, theta or absorbance) versus temperature for Cylindrospermopsis raciborskii (A, B, C) and Aphanizomenon ovalisporum (D, E, F) for either DNA amplified and melted in the presence of SYTO9 (A, D) or melting simulations conducted using the amplicon DNA sequences and either MELTSIM (B, E) or POLAND (C, F) respectively. The melt map for each sequence (base position versus predicted Tm) determined by MELTSIM is indicated by magenta circles. The equivalent profiles calculated by POLAND are indicated by blue plus signs or red squares for first order and second order reactions respectively.

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BMC Bioinformatics

ISSN: 1471-2105

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