Figure 4

Model discrimination based on two distinct steady states. The figure shows variations in v₁ simulated with model I (gray dashed line) and model II (black solid line), respectively. A large standard deviation of the experimental data (ε = 0.75, dashed error bars) has to be allowed for model I, otherwise mass balances are violated (ε = 0.25, solid error bars). For the simulations, the constants for complex formation were set to the following values: association k_on,i= 1; dissociation k_off = 0.1; catalytic step k_cat,i= 1. During the parametrization step, only the association constants of the complexes were estimated in a range from 0.3 to 3.