(A) An example TagSmart's graphical output. Every mutant is represented in 4 rows, representing signals from four tag-probe pairs (uptag-PM, uptag-cPM, downtag-PM, and downtag-cPM). The arrays are sequentially marked on the top row. The first two colored lines indicate the treatment and the time under which the mutant mixture was harvested and DNA was retrieved. The heatmap starts from the third colored line. Red indicates good fitness in treatment, and green indicates reduced fitness in treatment. (B) When mouse cursor is moved over the first two colored lines, the corresponding information for treatment and time will appear on screen. (C) When mouse cursor is over a dot in the heatmap, the actual fitness value will appear.