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Table 1 Comparison between serial dilution and LinReg for the measurement of efficiency

From: Statistical significance of quantitative PCR

Amplicon Test of equality* Test of variance**
Cav p = 0.24 p < 0.001
CTGF p < 0.001 p < 0.001
Eln p = 1 p < 0.001
FN p = 0.65 p < 0.001
L27 p = 0.8 p < 0.001
Perl p = 1 p < 0.001
PAI-1 p = 0.93 p < 0.001
  1. * Single one-way ANOVA was applied to the complete set of data to assess whether both the Serial dilution and the LinReg methods give similar averaged efficiencies (H0: efficiencies are equal, H1: efficiencies are different): a p-value below 0.05 indicates that measured efficiencies are significantly different. **F-test assessing whether the variance (error) induced by each method is the same [H0: variances are equal, H1: var(serial dilution) > var(LinReg)]: p < 0.001 indicates that the variance obtained with the serial dilution is significantly greater than that of LinReg.