Parameter | Df | F-value | p-value |
---|
Amplicon | 6 | 219.331 | <0.001 |
Sample | 10 | 7.227 | <0.001 |
Ct
| 1 | 0.646 | 0.422 |
Dilution | 4 | 2.111 | 0.078 |
Amplicon:Sample | 15 | 4.525 | <0.001 |
Amplicon:Ct | 6 | 1.957 | 0.07 |
Amplicon:Dilution | 20 | 2.576 | <0.001 |
Sample:Ct | 10 | 1.226 | 0.271 |
Sample:Dilution | 37 | 1.512 | 0.029 |
Ct: Dilution | 4 | 1.872 | 0.114 |
Residuals | 700 | | |
- A multiple ANOVA test was performed on efficiencies obtained from 700 reactions. Dependence with amplicon sequence, sample, Ct and dilutions were tested as well as all combinations of co-dependence. Df indicates the degree of freedom. The larger the F-value, or the smaller the p value, the more significant is the effect of the corresponding parameter.