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Table 2 Parameters influencing the efficiency of qPCR reactions.

From: Statistical significance of quantitative PCR

Parameter Df F-value p-value
Amplicon 6 219.331 <0.001
Sample 10 7.227 <0.001
Ct 1 0.646 0.422
Dilution 4 2.111 0.078
Amplicon:Sample 15 4.525 <0.001
Amplicon:Ct 6 1.957 0.07
Amplicon:Dilution 20 2.576 <0.001
Sample:Ct 10 1.226 0.271
Sample:Dilution 37 1.512 0.029
Ct: Dilution 4 1.872 0.114
Residuals 700   
  1. A multiple ANOVA test was performed on efficiencies obtained from 700 reactions. Dependence with amplicon sequence, sample, Ct and dilutions were tested as well as all combinations of co-dependence. Df indicates the degree of freedom. The larger the F-value, or the smaller the p value, the more significant is the effect of the corresponding parameter.