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Table 4 Resolution of each calculation model

From: Statistical significance of quantitative PCR

Relative concentrations ΔCT ECt (Savrg E)Ct (Pavrg E)Ct LR N0 Sigmoid Exponential
  t-value p-value t-value p-value t-value p-value t-value p-value t-value p-value t-value p-value t-value p-value
1 – 0.1 122.7 0.000 18.6 0.000 141.7 0.000 141.3 0.000 18.9 0.000 29.1 0.000 18.2 0.000
0.1 – 0.02 7.3 0.000 1.9 0.030 12.6 0.000 12.6 0.000 3.5 0.000 -4.4 N/A 0.8 0.225
0.02 – 0.01 0.7 0.232 0.4 0.360 1.6 0.052 1.6 0.052 0.9 0.190 6.1 0.000 -0.4 N/A
0.01 – 0.001 0.5 0.325 0.3 0.375 1.3 0.105 1.2 0.107 0.5 0.306 0.1 0.478 2.7 0.004
  1. A ANOVA coupled t-test was performed to determine which dilutions are statistically different from one another. The comparison of adjacent dilutions are shown. A p-value higher than 0.05 indicate that the model is unable to discriminate between the two adjacent dilutions. N/A stands for not available and indicate that the expected higher concentration of the comparison was in fact calculated to be lower from the qPCR results.