Relative concentrations

ΔCT

E^{Ct}

(Savrg E)^{Ct}

(Pavrg E)^{Ct}

LR N_{0}

Sigmoid

Exponential



tvalue

pvalue

tvalue

pvalue

tvalue

pvalue

tvalue

pvalue

tvalue

pvalue

tvalue

pvalue

tvalue

pvalue

1 – 0.1

122.7

0.000

18.6

0.000

141.7

0.000

141.3

0.000

18.9

0.000

29.1

0.000

18.2

0.000

0.1 – 0.02

7.3

0.000

1.9

0.030

12.6

0.000

12.6

0.000

3.5

0.000

4.4

N/A

0.8

0.225

0.02 – 0.01

0.7

0.232

0.4

0.360

1.6

0.052

1.6

0.052

0.9

0.190

6.1

0.000

0.4

N/A

0.01 – 0.001

0.5

0.325

0.3

0.375

1.3

0.105

1.2

0.107

0.5

0.306

0.1

0.478

2.7

0.004

 A ANOVA coupled ttest was performed to determine which dilutions are statistically different from one another. The comparison of adjacent dilutions are shown. A pvalue higher than 0.05 indicate that the model is unable to discriminate between the two adjacent dilutions. N/A stands for not available and indicate that the expected higher concentration of the comparison was in fact calculated to be lower from the qPCR results.