Destabilizing regions detection procedure. (a) clustering of highly destabilizing residues (red) that are less than 9.0 Å apart. (b) addition of destabilizing residues (orange) that are within 6.0 Å of a destabilizing residue already present in a destabilizing region. (c) Final result. Only the destabilizing regions larger than 4 residues are considered. In (a) and (b) are represented residues (cyan), destabilizing or not, which are enclosed in a sphere centered on a pair of destabilizing residues and therefore added to the destabilizing region (see text for more details).