Data Workflow. A. The protein of interest is digested with an enzyme and the resulting peptides are analyzed by LC MS. B. Peptide identity is then established with database search tools such as Sequest. C. The protein is incubated with D2O at multiple time points, digested with an enzyme and the resulting peptides are analyzed by LC MS. D. Resulting raw files are converted to mzXML. E. Search results are converted into PepXML. F. User selects the peptides of interest from the search results to create the peptide set. G. Core Deuterator processing and interactive tools for H/D/exchange data curation. H. Data visualization components such as graphs in Microsoft Excel and 3D structure overlays in PyMol.