Figure 7

Deuterium vs. Log Time (s). Differential H/D exchange data can be exported and plotted as %D vs. Log time (s) plots. Here we show data from a differential H/D exchange experiment to investigate perturbation in H/D exchange rates upon binding of a ligand (rosiglitazone) to PPARγ. Black line = PPARγ, Grey line = PPARγ + ligand. Top. The region between residues 279 and 287 exhibits a significant slow down in exchange rate following ligand binding. Bottom. Exchange rates for the region spanning residues 421–431 show no change. Statistical summary from a 2-way ANOVA between apo and ligand bound data; *** = P < 0.001, ns = not significant.