From: Establishing a major cause of discrepancy in the calibration of Affymetrix GeneChips
Calibration step | Option 1 | Option 2 | Option 3 | Option 4 | Option 5 |
---|---|---|---|---|---|
Background subtraction | Nothing | RMA | MAS | Â | Â |
Normalisation | Constant | Invariant set | Lowess | Qspline | Quantiles |
Expression Measure | Li-Wong (MBEI) | Median Polish (RMA) | MAS5 | Â | Â |