Figure 2

Combined view of the results from the search engines. The combined result view shows the comparison of 5 different search engines (SEQUEST, Mascot, Spectrum Mill, X! Tandem, and OMSSA) for bovine serum albumin (see experimental procedures for details). The line on the top lists the search results displayed in color. Sequence segments found only in one of the searches have the corresponding color whereas sequence segments found in multiple searches are colored red. The possible peptide modifications are shown under the protein sequence box. Three types of peptide modifications were defined: ICPL-light (K%), ICPL-heavy (K*), and oxidized methionine (MX$). X! Tandem generates additional modifications at the N-terminus (N-term@, N-term&, and N-term"). X! Tandem does not provide the possibility to search variable modification states on one amino acid. Therefore, for the X! Tandem search a fixed modification at K(+105.02) and a variable modification (K§6.02) has been applied. In the last table the peptides are listed and only one representative for the peptide at this modification state is shown.