From: A novel approach to sequence validating protein expression clones with automated decision making
Discrepancies defined on nucleotide and polypeptide levels | |
---|---|
Nucleotide changes | Polypeptide changes |
Substitution | Silent substitution |
 | Conservative substitution |
 | Non-Conservative substitution |
 | Missense substitution – any amino acid change |
 | Truncation – inframe Stop codon |
Frameshift deletion | Frameshift deletion |
Frameshift insertion | Frameshift insertion |
Inframe deletion | Inframe deletion |
Inframe insertion | Inframe insertion |
No Stop codon | Post-elongation |
No Start codon | No Translation |
Discrepancies defined on nucleotide level only | |
Discrepancies introduced by sequence ambiguity | |
   Start codon substitution | |
   Stop codon substitution | |
   Substitution in CDS region | |
   Frameshift insertion | |
   Inframe insertion | |
Discrepancies introduced by reference sequence ambiguity | |
   Substitution in CDS region | |
Flanking sequence region (5' and 3' regions described separately) | |
   Substitution – replacement of one base by another | |
   Deletion/insertion – deletion/insertion of several bases | |
   Ambiguous substitution – replacement of a base by ambiguous base | |
   Ambiguous insertion – insertion of ambiguous base | |
Discrepancy types for cDNA and flanking sequences defined at the nucleotide and polypeptide level by the Discrepancy Finder. |