Table 2 Comparison of automated vs. manual analysis on a sample plate of 93 unique genes
From: A novel approach to sequence validating protein expression clones with automated decision making
| Â | ACE | Researcher | ||
|---|---|---|---|---|
Analysis | Automatic + manual (20%) | Manual (100%) | ||
Samples | 93 | 93 | ||
Clones for: | Samples | Time | Samples | Time |
End Read Analysis | 93 | 45 min | 93 | 270 min |
Internal Analysis | 45 | 30 min | 45 | 100 min |
Special Handling# | 20 | 65 min | 1 | 5 min |
Time Total | 93 | 140 min | 93 | 275 min |
Outcome | Clones | % | Clones | % |
Accepted Clones | 73 | 78.5% | 73 | 78.5% |
Rejected Clones | 17 | 18.3% | 13 | 14.0% |
Pending Clones | 3 | 3.2% | 7 | 7.5% |