A survey of orphan enzyme activities

BMC Bioinformatics

Table 11 Selected salvageable orphans

EC No.	Ranking	Pathways	Activity	Species	Full Genome Sequence?	Ongoing Genomic Sequencing?	Mr (kDa)	pI (pH units)
3.5.1.30	Good	None	5-amino-penta-namidase	Pseudomonas putida P2, Pseudomonas fluorescens	Yes (P. putida)*	Several Pseudomonas species	67	N/A
5.5.1.11	Good	None	Dichloro-muconate cyclo- isomerase	Alcaligenes eutrophus JMP 134 (Ralstonia eutropha JMP134)	N/A	Yes	40 ± 10	N/A
4.2.1.97	Marginal	None	Phaseollidin hydratase	Fusarium solani f.sp. Phaseoli	No	Different species (Fusarium sporotrichioides)	monomer 1: 47 monomer 2: 49
2.3.1.103	Poor	None	Sinapoylglucose–sinapoylglucose O-sinapoyltransferase	Raphanus sativus	N/A	N/A	55	N/A

A selection of orphans with different salvageability rankings are listed. Pathway names are those used in the MetaCyc database. *: The genomes of several strains of P. fluorescens are in the final stages of assembly and are essentially fully sequenced. N/A: not available

ISSN: 1471-2105