Name of enzyme activity
Is lack of sequence confirmed?
Bibliographical data (publication dates, authors, institutions)
Name of species
Can the species associated with the original publications be unambiguously identified?
Is a comprehensive genome sequence available for those species?
Are comprehensive genome sequences available from closely-related species?
Is there ongoing genomic sequencing for those species or from closely-related species?
Are molecular data such as Mr and pI available?
Does the purification and characterization procedure suggest that purifying this enzyme should be reasonably straightforward?