Table 3 32 newly identified genes with highly conserved uORFs strongly predicted by the rule set to be functional (marked in bold), with an optimal spacing to the main ORF and other uORFs. Numbering of uORFs is 3' to 5', as uORFs were found from intergenic sequences.
ORF | Name | Length (nt) of intergenic sequence | Number and size (codons) of uORFs | Position (nt) relative to start codon of main ORF | Predicted length (nt) of 5'-UTR [20]a |
|---|---|---|---|---|---|
YDL146W | LDB17 | 489 | uORF1(3) | -53 | 514 |
YDL176W | Uncharacterised | 376 | uORF1(9) | -104 | 157 |
YDL205C | HEM3 | 860 | uORF1(8) | -130 | 183 |
YEL061C | CIN8 | 509 | uORF1(4) | -109 | 128 |
YER167W | BCK2 | 757 | uORF1(7) | -245 | 286 |
YGL006W | PMC1 | 387 | uORF1(4) | -144 | 438 |
YJL139C | YUR1 | 505 | uORF1(8) | -55 | 60 |
YKL182W | FAS1 | 1030 | uORF1(6) | -142 | 548 |
YLR047C | FRE8 | 825 | uORF1(3) | -230 | 264 |
YLR427W | MAG2 | 315 | uORF1(6) | -71 | 109 |
YMR145C | NDE1 | 1006 | uORF1(4) | -165 | 215 |
YNL053W | MSG5 | 358 | uORF1(4) | -104 | 120 |
YNL094W | APP1 | 771 | uORF1(3) | -155 | 278 |
YNR016C | ACC1 | 1539 | uORF1(3) | -342 | 540 |
YOL100W | PKH2 | 1317 | uORF2(9) uORF1(7) | -338 -81 | 255 |
YOL130W | ALR1 | 1042 | uORF1(5) | -103 | 290 |
YOR061W | CKA2 | 371 | uORF1(5) | -103 | 162 |
YOR124C | UBP2 | 388 | uORF1(7) | -211 | 250 |
YOR137C | SIA1 | 628 | uORF1(9) | -367 | 440 |
YOR231W | MKK1 | 488 | uORF1(9) | -72 | 148 |
YOR254C | SEC63 | 248 | uORF1(5) | -82 | 100 |
YPL057C | SUR1 | 373 | uORF1(5) | -145 | 253 |
YPR026W | ATH1 | 821 | uORF1(4) | -65 | 112 |
YER118C | SHO1 | 441 | uORF1(8) | -212 | 206 |
YEL013W | VAC8 | 541 | uORF2(3) uORF1(3) | -364 -306 | 252 |
YEL026W | SNU13 | 692 | uORF3(3) uORF2(2) uORF1(2) | -206 -198 -193 | 170 |
YLR009W | RLP24 | 454 | uORF1(3) | -220 | 171 |
YLR243W | Uncharacterised | 320 | uORF1(3) | -111 | 80 |
YML093W | UTP14 | 241 | uORF1(4) | -99 | 21 |
YMR215W | GAS3 | 313 | uORF1(4) | -147 | 62 |
YNL229C | URE2 | 457 | uORF2(11) uORF1(5) | -384 -285 | 217 |
YNR049C | MSO1 | 393 | uORF1(3) | -142 | 123 |