Table 4 Calculated minimum free folding energy of the 200 nt immediately upstream of the start codon of different sets of uORF-containing genes [21].

9 genes in initial set with conserved uORFs (Table 1; Fig. 2 set C)

-25.8

32 genes with highly conserved uORFs with optimal spacing (Table 3; Fig. 2 set H)

-32.8

252 genes with highly conserved uORFs (additional file 2; Fig. 2 set G)

-35.4

All genes in genome

-36.6