Figure 2

In this figure, interactions identified by analysis but not previously reported are observed to have potential biological interest. Genes and their coded proteins are ovals, arrows are identified, directed interactions, and the purple box includes those proteins that comprise histone complexes. All histone genes are identified to concurrently regulate other genes, correctly identifying HTA2, HTA1, HTB1, HTB2, HHT1, and HHF1 as a single functional unit. All regulated genes are associated with DNA-interacting proteins, which are logical functional partners to histone complexes. Regulated genes are: HEK2, associated with the regulation of telomeres [44, 45]; HTZ1, concerned with transcriptional regulation through heterochromatin structure [46]; SAS3, a cell cycle related histone acetyltransferase that is involved in transcriptional regulation [47]; SGS1, involved in maintenance of genome integrity and regulates chromosome synapsis and meiotic crossing over [48, 49]; SIM1, a cell cycle-regulated DNA replication gene [50]; ALK1, a cell cyle-regulated protein kinase involved with the response to DNA damage [51]; FPR4, a nuclear protein with GO annotations that include chromatin and histone associations [52-54]; and YPL141C, an unknown protein. The presence of YPL141C here however, suggests that its function may be related to chromatin structure, or cell cycle regulation.