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Table 1 The difference in average BPP values and G+C content in 6th – 30th CDS positions of human and mouse cDNAs characterized by optimal and suboptimal start codon contexts (purine (Pu) and pyrimidine (Py) in pos. -3 upstream AUG, respectively)†

From: AUG_hairpin: prediction of a downstream secondary structure influencing the recognition of a translation start site

CDS pos.

human

mouse

 

BPPPy-BPPPu

GCPy-GCPu,%

BPPPy-BPPPu

GCPy-GCPu,%

6

-0.014

-3.93

0.007

-1.16

7

0.005

-2.23

-0.016

-3.14

8

0.011

1.34

-0.002

0

9

0.006

-2.36

-0.006

-1.07

10

0.006

1.15

-0.014

-1.21

11

-0.008

2.14

-0.004

-1.47

12

-0.007

-3.79

0.005

2.46

13

0.015*

-0.41

-0.004

-0.38

14

0.012**

1.65

0.020*

4.47

15

0.015***

-1.99

0.018**

-1.08

16

0.014*

-0.93

0.036***

-1.48

17

0.012*

4.21

0.019*

7.53

18

-0.003

-3.23

-0.005

-5.46

19

0.004

0.59

0.010

1.31

20

0.005

0.68

-0.008

2.8

21

-0.025

-4.94

-0.012

-1.29

22

-0.005

-0.50

-0.013

-1.6

23

-0.018

3.79

-0.020

5.15

24

-0.020

-3.51

0.001

-3.21

25

0.007

-0.70

-0.013

-0.32

26

0.017*

2.14

-0.003

4.01

27

0.006

-3.66

-0.022

-4.23

28

-0.001

-0.03

-0.022

1.02

29

0.007

5.30

-0.022

5.21

30

-0.016

-3.68

-0.035

-5.06

  1. † CDS positions where mean BPP values were significantly higher in mRNA samples with a suboptimal start codon context are marked (BPPPy-BPPPu values are bolded; the significance level (according to Mann-Whitney U-test) is shown by numbers: * p < 0.05; ** p < 0.01;*** p < 0.001)