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Table 2 The difference in characteristics of 5'-end proximal hairpins started in 6th – 20th CDS positions of human and mouse cDNAs characterized by optimal and suboptimal start codon contexts (purine (Pu) and pyrimidine (Py) in pos. -3 upstream AUG, respectively; Etot, energy of an eligible hairpin, kcal/mol; Est, energy of the hairpin stem region, kcal/mol; Lst, size of the hairpin stem region, base pairs)†

From: AUG_hairpin: prediction of a downstream secondary structure influencing the recognition of a translation start site

CDS pos.

human

mouse

 

EtotPy-EtotPukcal/mol

EstPy-EstPu kcal/mol

LstPy-LstPu b.p.

EtotPy-EtotPu kcal/mol

EstPy-EstPu kcal/mol

LstPy-LstPu b.p.

6

0.45

0.21

-0.14

0.09

-0.16

0.08

7

0.40

0.38

-0.19

0.02

0.35

-0.26

8

-0.25

0.15

-0.22

1.04

-0.027

-0.16

9

1.79

0.11

-0.203

0.24

-0.53

0.02

10

1.89

0.01

0.13

-0.47

-0.29

0.11

11

0.79

0.21

-0.09

-0.87

-0.80

0.16

12

1.12

0.11

-0.02

0.86

1.29

-0.64

13

-1.28*

0.39

-0.13

-3.65*

-1.02*

0.19

14

-2.41*

-1.09**

0.10

1.12

1.65

-0.74

15

-0.87

-0.29

0.09

-1.84

0.54

-0.47

16

-1.03**

-0.53*

0.26*

3.42

-0.10

0.11

17

2.87

-1.15**

0.71*

-3.73*

-0.60*

0.46

18

-0.94

0.13

-0.24

3.08

-0.43

0.04

19

-2.99*

0.82

-0.48

1.88

0.75

0.10

20

-1.82

-0.58

0.06

-2.74

0.47

-0.32

  1. † CDS positions where mean Etot and Est values were significantly lower (and Lst values were significantly higher) in mRNA samples with a suboptimal start codon context are marked (EtotPy – EtotPu, EstPy – EstPu, and LstPy – LstPu values are bolded; the significance level (according to Mann-Whitney U-test or Kolmogorov-Smirnov two-sample test) is shown by numbers: * p < 0.05; ** p < 0.01;*** p < 0.001)