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Figure 1 | BMC Bioinformatics

Figure 1

From: Optimization of filtering criterion for SEQUEST database searching to improve proteome coverage in shotgun proteomics

Figure 1

Distribution of peptides identified from human liver tissue lysate by SEQUEST. A) Singly charged peptides; B) Doubly charged peptides; C) Triply charged peptides. Each data point represents a peptide identification from the composite database: cross represents peptide identification from reversed sequence while square indicates peptide identification from forward sequence. Cumulate curves drawn in each graph are 1% false-discovery curves. Each point on curves indicates a filtering criterion leading to peptide identification with FDR of 1%, and the identified peptides by each criterion present in the region where Xcorr and ΔCn scores are higher than the Xcorr and ΔCn cutoffs in each set of criteria. Graphs were drawn using the Speed Model by Origin 7.5 with 5000 max points per curve, and three raw graphs with all data points were shown [see Additional file 1].

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