The influence of length on run-time. Comparison of the five programs processing increasingly longer windows of a set of five rRNA SSU sequences. Note that RNAspa was run in Boltzmann sampling mode across all sequence lengths. All but RNAspa failed to run on sequences greater than 450 bps due to memory constraints. StemLoc does not appear in the graph because it failed to process sequences of 100 bps or more. As expected, a cubic trendline (not shown) fits RNAspa's curve with the R2 value of 0.9965. RNAspa gave a MCC score of 0.34 for the complete ~1,800 bps long SSU family.