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Table 2 Kinetic constants for triose phosphate isomerases (TPI) from 12 different organisms with glyceraldehyde-3-phosphate as substrate

From: qPIPSA: Relating enzymatic kinetic parameters and interaction fields

Organism

Km (mM)*

kcat (105 min-1)

kcat/Km (107 M-1s-1)

SwissProt ID Sequence identity to 1r2rA

Conditions, Reference

Trypanosoma brucei

0.46 (0.25–0.46)

3.1 (2.6–3.7)

1.120

TPIS_TRYBB 53%

20°C, 100 mM, pH 7.4 [56]

Trypanosoma cruzi

0.45

2.7

1.000

TPIS_TRYCR 53%

20°C, 100 mM, pH 7.4 [57]

Oryctolagus cuniculus muscle

0.43 (0.32–0.43)

1.86 (1.9–5.1)

0.721

TPIS_RABIT 100%

30°C, 50 mM, pH 7.6 [56]

Gallus gallus muscle

0.47

2.56

0.915

TPIS_CHICK 89%

30°C, 100 mM, pH 7.4 [58]

Saccharomyces cerevisiae

1.22 (0.62–1.5)

7.9 (1.4–10))

1.080

TPIS_YEAST 52%

25°C, 100 mM, pH 7.6 [59]

Leishmania mexicana

0.41 (0.30–0.41)

2.52 (4.3–2.5)

1.020

TPIS_LEIME 52%

25°C, 100 mM, pH 7.6 [60]

Plasmodium falciparum

0.35

2.68

1.280

TPIS_PLAFA 42%

30°C, 50 mM, pH 7.9 [56]

Vibrio marinus

1.9

4.2

0.368 (0.515)#

TPIS_VIBMA 41%

10°C, 100 mM, pH 7.6 [61]

Escherichia coli

1.03

5.4

0.874

TPIS_ECOLI 45%

25°C, 100 mM, pH 7.6 [61]

Homo Sapiens

0.49

2.7

0.918

TPIS_HUMAN 98%

25°C, 100 mM, pH 7.6 [62]

Giardia lamblia

0.53

2.9

0.912

TPI1_GIALA 45%

25°C, 100 mM, pH 7.4 [63]

Spinacia oleracea

0.68

2.7

0.662

TPIC_SPIOL 60%

25°C, 100 mM, pH 7.5 [64]

  1. * The range of values extracted from BRENDA is given in brackets.
  2. # The value after correction to 25°C obtained by multiplying by 1.4 (the ratio of water viscosity at 10°C compared to 25°C).