Figure 3
From: Single TNFα trimers mediating NF-κ B activation: stochastic robustness of NF-κ B signaling
Pulse-Pulse experiment. Panels A to F: IKKa and NF-κ B oscillation amplitude resulting from single cell numerical simulations. Two TNFα (20 ng/ml), 5 min pulses are separated by 30', 60', 90' 120' 150' or 180' breaks. Four single cell simulations (marked by different colors) are shown in each panel. Panel G: IKK kinase activity in 3T3 cells stimulated by two 5 min. pulses of TNFα (20 ng/ml), separated by 30', 60', 90' 120' 150' or 180' break. First sub-blot corresponds to unstimulated cells, second sub-blot shows intensity of first peak, while next six sub-blots show the intensity of second peak as a function of brake duration, see Additional file 1 for the protocol of the experiment. Panels H and I magnitude of the second and the first peak of IKK and NF-κ B activity (nuclear NF-κ B and IKKa) calculated from average over 500 cells. In numerical experiment presented in Panel I, the receptor activity coefficient k a was elevated 10 fold before second TNFα pulse.