Figure 7

Average IKK and NF-κ B activity (nuclear NF-κ B and IKKa) as function of TNFα dose, model versus Cheong et al. 2006 experiment [45]. Panels A: IKKa and IKK kinase activity, Panel B: nuclear NF-κ B calculated as average of N single cell simulation: N = 50 for TNFα dose equal 10 ng/ml, N = 100 (1 ng/ml), N = 500 (0.1 ng/ml) and N = 2500 (0.01 ng/ml). and EMSA assays of nuclear NF-κ B. To compare our predictions to experimental data, we rescale the time coordinate, i.e. we calculate the values of corresponding functions in the same time points as in the experiment. Despite the qualitative agreement between the model and the experiment shown there is substantial difference in IKK activity peak timing and smaller difference in NF-κ B profile timing for 10 ng/ml dose. In fact there is also large discrepancy between different experiments; here the IKK activity peak is observed for 5 min., while in [50] for 10 min. and in [62] for 15 min.