LeuTAa | rGAT1 | rSERT | hDAT | Structural location | Description | Experimental evidence | Predicted by2 |
---|---|---|---|---|---|---|---|
(a.) Substrate/Na + -binding sites | |||||||
G20 | G59 + | G94 + | G75 | TM1a | Na+ binding site | [2–95] | P,S,R,E |
N21 | Y60 + | Y95 + | F76 + | TM1a | Leu binding site | [94–99] | None |
A22 | A61 | A96 | A77 | TM1a | Leu & Na+ binding sites | None | F,R |
V23 | I62 + | V97 | V78 | Unwound TM1 | Na+ binding site | [95] | F |
G24 | G63 + | D98 + | D79 + | Unwound TM1 | Leu binding site | [95, 97, 100, 101] | F,R,E,D |
L25 | L64 + | L99 + | L80 + | TM1b | Leu binding site | [92, 95, 102] | P,F,S,R,E |
G26 | G65 + | G100 + | A81 | TM1b | Leu binding site | [92, 95] | P,F,S,R |
N27 | N66 + | N101 + | N82 | TM1b | Na+ binding site | [92, 95] | P,F,S,R,E |
E62 | E101 + | E136 + | E117 + | TM2 | Stabilizes TM6 unwound region | [55–58] | P,F,S,R,E |
V104 | L136 - | I173 + | V152 + | TM3 | Leu binding site | [93, 96, 103–105] | None |
Y108 | Y140 + | Y176 + | Y156 + | TM3 | Leu binding site | [106–108] | F,S,R |
F252 | F293 | F334 | C319 + | TM6a | Leu binding site | [109] | P,F,S,R |
F253 | F294 + | F335 + | F320 + | TM6a | Leu binding site | [93, 98, 103, 110] | P,F,S |
T254 | S295 + | S336 | S321 + | TM6a | Leu & Na+ binding sites | [108, 110, 111] | P,F,E |
S256 | G297 | G338 | G323 + | Unwound TM6 | Leu binding site | [112] | P,F,E |
L257 | L298 + | P339 + | V324 | Unwound TM6 | Leu binding site | [110, 113] | P |
G258 | G299 | G340 | G325 + | Unwound TM6 | Leu binding site | [98] | P |
F259 | L300 | F341 + | F326 - | Unwound TM6 | Leu binding site | [93, 98, 103] | P |
G260 | G301 | G342 | G327 + | Unwound TM6 | Leu binding site | [112] | P,S,E |
N286 | N327 + | N368 | N353 | TM7 | Na+ binding site | [111, 114] | P,F,R |
A351 | L392 | L434 | L418 | TM8 | Na+ binding site | None | F,R,E,D |
T354 | D395 + | D437 | D42 + | TM8 | Na+ binding site | [111, 115] | P,F,R,E,D |
S355 | S396 + | S438 + | S422 | TM8 | Leu & Na+ binding sites | [93, 103, 111] | P,F,S,D |
I359 | T400 | G442 + | G426 | TM8 | Leu binding site | [93, 103] | None |
(b.) Cytoplasmic gate | |||||||
R5 | R44 + | R70 | R60 + | Â | Cytoplasmic gate | [116] | S |
W8 | W47 + | W82 | W63 + | Â | Cytoplasmic gate | [58, 116] | S |
S267 | S308 | S349 | S334 | Â | Cytoplasmic gate | None | S,R,E |
Y268 | Y309 - | Y350 | Y335 + | Â | Cytoplasmic gate | [57, 106, 115] | S,E |
D369 | D410 | D452 | D436 + | Â | Cytoplasmic gate | [58, 115] | S,E |
(b.) Extracellular/periplasmic gate | |||||||
R30 | R69 + | R104 + | R85 + | TM1b | Extracellular gate | [57, 92, 95, 97, 117] | P,F,S,R,E |
Y47 | Y86 + | Y121 | Y102 + | TM2 | Extracellular gate | [106, 108] | P,F,S,R,E |
Q250 | Q291 + | Q332 | Q317 + | TM6a | Extracellular gate | [57, 108, 110] | P,F,S,R,E |
E290 | S331 + | S372 | L355 | TM7 | Extracellular gate | [111, 114] | P |
D404 | D451 | E493 + | D476 + | TM10 | Extracellular gate | [58, 104] | P,S |