Table 1 Classes of genes and GSTs and class description

GST classification

GST5

Uniquely tagging

The GST primary BLAST hit overlaps with exon(s) of an annotated gene over at least 100 bp and none of the GSTs BLAST hits significantly overlap with exon(s) of another gene.

GST4

Dubiously tagging

The GST primary BLAST hit overlaps with exon(s) of an annotated gene over at least 100 bp, the primary BLAST hit doesn't share 30 bp with exon(s) of another gene, but the GST has a secondary BLAST hit having more than 70% sequence identity with exon(s) of another gene.

GST3

Co- tagging

The GST primary BLAST hit overlaps with exon(s) of an annotated gene over at least 100 bp, but the primary BLAST hit also shares at least 30 bp with exon(s) of another gene.

GST2

Insufficiently tagging

The GST primary BLAST hit overlaps with exon(s) of an annotated gene, but over less than 100 bp.

GST1

Not tagging

The GST primary BLAST hit overlaps with no annotated exon.

Gene classification

GE5

Uniquely tagged

At least one GST tags this gene uniquely, according to the definition of GST5.

GE4

Dubiously tagged

At least one GST tags this gene dubiously, according to the definition of GST4. No GST tags this gene uniquely.

GE3

Co-tagged

At least one GST co-tags this gene, according to the definition of GST3. No GST tags this gene dubiously or uniquely.

GE2

Insufficiently tagged

At least one GST tags this gene insufficiently, according to the definition of GST2. No GST co-tags this gene, nor tags this gene dubiously or uniquely.

GE1

Not tagged

The primary BLAST hit of no single GST overlaps with exon(s) of this gene.