From: Absolute estimation of initial concentrations of amplicon in a real-time RT-PCR process
Cyp1B1 means of 3 estimates with std error | |||||
---|---|---|---|---|---|
 | basic model | expanded model |  | ||
true fold change | 40 cyc | 60 cyc | 40 cyc | 60 cyc | Livak et al |
1e6/1e5 = 10 | 11.5 (0.504) | 11.3 (0.374) | 11.3 (0.389) | 11.3 (0.587) | 14.0 (1.08) |
1e6/1e4 = 100 | 121.6 (4.91) | 115.2 (4.00) | 133.2 (4.48) | 117.1 (4.59) | 112.2 (4.81) |
1e6/1e3 = 1000 | 1133 (19.83) | 1049 (23.4) | 1170 (21.4) | 1066 (10.78) | 1267 (30.9) |
RNaseP means of 4 estimates with std error | |||||
 | basic model | expanded model |  | ||
true fold change | 40 cyc | 60 cyc | 40 cyc | 60 cyc | Livak et al |
2e4/1e4 = 2 | 2.2 (.039) | 2.2 (.098) | 2.6 (0.278) | 2.0 (0.078) | 2.0 (.043) |
2e4/5e3 = 4 | 4.2 (.0164) | 4.0 (.032) | 5.2 (0.44) | 4.7 (0.114) | 4.1 (.083) |
2e4/2.5e3 = 8 | 8.4 (.142) | 7.5 (0.117) | 10.3 (0.677) | 9.2 (0.299) | 9.0 (.0678) |
2e4/1.25e3 = 16 | 18.3 (0.295) | 16.4 (0.681) | 22. (1.83) | 19.7 (0.434) | 19.1 (.419) |