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Table 3 Comparison of fold change estimates for Cyp1B1 and RNaseP

From: Absolute estimation of initial concentrations of amplicon in a real-time RT-PCR process

Cyp1B1 means of 3 estimates with std error

 

basic model

expanded model

 

true fold change

40 cyc

60 cyc

40 cyc

60 cyc

Livak et al

1e6/1e5 = 10

11.5 (0.504)

11.3 (0.374)

11.3 (0.389)

11.3 (0.587)

14.0 (1.08)

1e6/1e4 = 100

121.6 (4.91)

115.2 (4.00)

133.2 (4.48)

117.1 (4.59)

112.2 (4.81)

1e6/1e3 = 1000

1133 (19.83)

1049 (23.4)

1170 (21.4)

1066 (10.78)

1267 (30.9)

RNaseP means of 4 estimates with std error

 

basic model

expanded model

 

true fold change

40 cyc

60 cyc

40 cyc

60 cyc

Livak et al

2e4/1e4 = 2

2.2 (.039)

2.2 (.098)

2.6 (0.278)

2.0 (0.078)

2.0 (.043)

2e4/5e3 = 4

4.2 (.0164)

4.0 (.032)

5.2 (0.44)

4.7 (0.114)

4.1 (.083)

2e4/2.5e3 = 8

8.4 (.142)

7.5 (0.117)

10.3 (0.677)

9.2 (0.299)

9.0 (.0678)

2e4/1.25e3 = 16

18.3 (0.295)

16.4 (0.681)

22. (1.83)

19.7 (0.434)

19.1 (.419)

  1. The estimates of the initial amounts of amplicon from Tables 1 and 2 were used to estimate the fold change from the solutions with the largest initial amounts of amplicon to those with lower amounts. The first column gives the correct fold change. The final column shows fold change estimates using the method described by Livak et al [3](See text.).