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Table 4 Estimates from averaged data

From: Absolute estimation of initial concentrations of amplicon in a real-time RT-PCR process

 

Cyp1B1.

Actin

 

AmpliconCount

Basic Model

Expanded Model

Basic Model2

Expanded Model3

ABI Master Mix primer = 400 nmol/L probe = 400 nmol/L

103

0.78 × 103

0.68 × 103

0.25 × 103

0.24 × 103

 

104

0.71 × 104

0.64 × 104

0.36 × 103

0.35 × 103

 

105

0.86 × 105

0.77 × 105

0.48 × 104

0.47 × 104

 

106

1.0 × 106

0.94 × 106

0.59 × 105

0.57 × 105

ABI Master Mix primer = 400 nmol/L probe = 200 nmol/L

103

0.48 × 103

0.52 × 103

0.26 × 103

0.28 × 103

 

104

0.48 × 104

0.52 × 104

0.39 × 104

0.41 × 104

 

105

0.62 × 105

0.64 × 105

0.37 × 105

0.40 × 105

 

106

0.77 × 106

0.78 × 106

0.45 × 106

0.47 × 106

ABI Master Mix primer = 200 nmol/L probe = 400 nmol/L

103

0.50 × 103

0.48 × 103

0.27 × 103

0.23 × 103

 

104

0.54 × 104

0.46 × 104

0.46 × 104

0.38 × 104

 

105

0.76 × 105

0.70 × 105

0.53 × 105

0.48 × 105

 

106

0.90 × 106

0.86 × 106

0.67 × 106

0.58 × 106

ABI Master Mix primer = 200 nmol/L probe = 200 nmol/L

103

0.33 × 103

0.32 × 103

0.20 × 103

0.20 × 103

 

104

0.34 × 104

0.33 × 104

0.26 × 104

0.26 × 104

 

105

0.42 × 105

0.42 × 105

0.30 × 105

0.29 × 105

 

106

0.53 × 106

0.53 × 106

0.39 × 106

0.38 × 106

  1. Estimation results of initial amounts of Cyp1B1 and Actin amplicons. Initial concentrations of probe and primer are given in the first column. Note that using the extended model only increases the estimates for the PCRs with a higher initial primer concentration than probe. 1Initial number of amplicon molecules placed in solution. 2Equations found in Table 2. 3Equation (S1) in the Appendix.