Figure 8

Schematic diagram for detecting insertion and nested insertion using the ancestral structural consensus. A. A phylogenetic tree rooted with an outgroup domain. d₁ ~ d₅ represent the homologous domains in a domain family, and n₀ ~ n₃ represent the ancestral structural consensus. B. Detect insertion and nested insertion using the aligned character sequences of the ancestral structural consensus, which are grouped together according to evolutionary stages from n₀ to d₅. In the aligned sequences, a site with 'A' denotes over 90% structures of all descendant domains have aligned residues at this site, otherwise the site is denoted as a gap '-'. var1, var2, and var3 are three insertion-containing regions determined with the aligned sequences, in which var2 is a variable region with nested inserts.