Misclassifications in dataset I. Misclassified genes in dataset I by ArrayLeaRNA (A), OpWise (B) and the two fold cut-off (C). The features unique to the strains NCTC 81116 (△) and NCTC 11168 (□) and misclassified as equally described by ArrayLeaRNA and the two fold cut-off showed intensities typical of equally transcribed genes. Only features unique to the strains NCTC 11168 (□) could be analysed by OpWise. The features classified as equally expressed (○) are also shown for each approach. The cut-off values for the posterior probability of equal expression were 0.01 for ArrayLeaRNA and 0.99 and 0.01 for OpWise.