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Table 1 Hammerhead ribozymes targeted to 15 GUC cleavage sites in the BCRP mRNA

From: A structural analysis of in vitro catalytic activities of hammerhead ribozymes

Ribozymea Position of target site b Activity [(1-Su3600) ± SD] ΔGdisruption(kcal/mol) ΔGtotal (kcal/mol)
GUC1 -174 0.843 ± 0.047 -15.958 -5.423
GUC2 -131 0.890 ± 0.045 -11.898 -13.288
GUC3 +3 0.811 ± 0.116 -2.466 -17.571
GUC4 +16 0.803 ± 0.062 -11.090 -4.677
GUC5 +36 0.876 ± 0.030 -10.879 -9.883
GUC6 +164 0.802 ± 0.053 -17.339 -2.715
GUC7 +221 0.934 ± 0.025 -11.197 -16.294
GUC8 +274 0.844 ± 0.027 -7.312 -13.835
GUC9 +490 0.803 ± 0.030 -17.625 -6.215
GUC10 +506 0.829 ± 0.028 -15.457 -8.610
GUC11 +558 0.664 ± 0.067 -10.178 -26.245
GUC14 +1109 0.788 ± 0.066 -13.385 -5.469
GUC17 +1201 0.914 ± 0.067 -8.426 -11.931
GUC18 +1207 0.920 ± 0.042 -5.473 -17.366
GUC19 +1398 0.940 ± 0.047 -7.868 -9.736
  1. aRibozymes GUC12, GUC13, GUC15 and GUC16 were not tested.
  2. bPosition of G of the GUC cleavage site in the ABCG2 cDNA relative to the A of the ATG start codon (GenBank accession no. NM_004827).