Table 1 Hammerhead ribozymes targeted to 15 GUC cleavage sites in the BCRP mRNA
From: A structural analysis of in vitro catalytic activities of hammerhead ribozymes
Ribozymea | Position of target site b | Activity [(1-Su3600) ± SD] | ΔGdisruption(kcal/mol) | ΔGtotal (kcal/mol) |
|---|---|---|---|---|
GUC1 | -174 | 0.843 ± 0.047 | -15.958 | -5.423 |
GUC2 | -131 | 0.890 ± 0.045 | -11.898 | -13.288 |
GUC3 | +3 | 0.811 ± 0.116 | -2.466 | -17.571 |
GUC4 | +16 | 0.803 ± 0.062 | -11.090 | -4.677 |
GUC5 | +36 | 0.876 ± 0.030 | -10.879 | -9.883 |
GUC6 | +164 | 0.802 ± 0.053 | -17.339 | -2.715 |
GUC7 | +221 | 0.934 ± 0.025 | -11.197 | -16.294 |
GUC8 | +274 | 0.844 ± 0.027 | -7.312 | -13.835 |
GUC9 | +490 | 0.803 ± 0.030 | -17.625 | -6.215 |
GUC10 | +506 | 0.829 ± 0.028 | -15.457 | -8.610 |
GUC11 | +558 | 0.664 ± 0.067 | -10.178 | -26.245 |
GUC14 | +1109 | 0.788 ± 0.066 | -13.385 | -5.469 |
GUC17 | +1201 | 0.914 ± 0.067 | -8.426 | -11.931 |
GUC18 | +1207 | 0.920 ± 0.042 | -5.473 | -17.366 |
GUC19 | +1398 | 0.940 ± 0.047 | -7.868 | -9.736 |