Figure 1

PSD sequence data and metadata. This is an example web page from the PSD containing all data and metadata associated with a protein sequence (DNA sequence pages are identical). A) Data entered by the user, either manually or automatically during batch import, and BLASTAgent settings. Notice that this particular sequence is not enrolled in BLASTAgent, but a BLASTP search of NCBI NR database has been run and the 596 hits are stored for the user to view. BLAST search options are illustrated; for example, the Entrez term "Caenorhabditis elegans [orgn]" limits the database hits to only those from C. elegans. B) Protein domains. Potential protein domains can be identified for any PSD sequence. A BLAST search of the NCBI CDD database is run and hits are illustrated graphically and by text. C) Sequence metadata. Sequence-associated data may be generated by tools integrated within the PSD, as in the top 2 entries, a BLAST2 alignment and a ClustalW multiple sequence alignment, or from external tools as the last entry illustrates.