Figure 7

Multiple sequence alignment of selected representatives of SPOUT COGs. Sequences are denoted by the COG number, species' name, the NCBI gene identification (GI) number and the PDB code (if applicable). The variable termini and non-conserved insertions are not shown; the number of omitted residues is indicated in parentheses. Amino acids are colored according to the physico-chemical properties of their side-chains (negatively charged: red, positively charged: blue, polar: green, hydrophobic: grey). The consensus secondary structure is shown above the alignment as tubes (helices) and arrows (strands). The most typical positions of AdoMet-binding residues are indicated above the alignment by vertical red arrows, while the typical positions of catalytic residues are indicated by blue arrows. Note that additional catalytic residues may be present in the N-terminal part (unalignable and therefore not shown in this figure) and that the position of catalytic residues varies between families, e.g. it depends on the mode of dimerization.