Figure 3

Interactive SpliceMiner query results. This figure is a composite of five separate interactive queries. Each query corresponds to a different Affymetrix HG-U133A Probe. The composite permits facile comparison of the exons that are targeted by each of the probes. For example, the probes for exons 16 and 18 uniquely identify the splice variants NM_006487 and NM_006485 respectively. In contrast, the probe for exon 17 identifies an unspecified mixture of splice variants BC022497 and NM_001996. Although that probe does not provide unique identification, it reduces the ambiguity from 7 splice variants to 2 splice variants. A somewhat more complex identification is afforded by the combined use of the two probes for exon 24. By difference, those probes in theory can uniquely identify the splice variant U01244. Variants are identified by accession ID, and a map of the exons in each variant is displayed. Exons are indicated by larger blue bars and are drawn to scale. Thin blue lines represent intron sequences but are not drawn to scale. If the query consists of a sequence or set of coordinates, a red vertical line identifies the matching location. A pop-up tool tip displays the URL query string that is invoked upon clicking on an accession ID. Another pop-up tool tip, which provides the exact genomic coordinates of the exon, is displayed by mousing-over the exon.