Figure 4From: TAMEE: data management and analysis for tissue microarraysStained and gridded TMA section. (a) TMA section with about 480 tissue cores from different cancer tissue samples. Cytokeratin has been detected by IHC (brown areas) using a cytokeratin antibody (mouse anti human; DAKO; M3515) and the EnVision detection Kit (DAKO, K4065). Tissue cores are made visible with a Hematoxilin counterstain (light blue). (b) The same section after automatic core localization and shape determination. The outline of a core is displayed in blue. Positions with missing cores (⊗), cores which are too large (more than 160% of the expected area, ) and cores which are too small (less than 40% of the expected area, ) are shown in red. Array positions without a symbol or outline have been deliberately left empty during TMA production. Gridding ambiguities in heavily distorted areas of the array are corrected manually.Back to article page