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Table 2 Analysis of real-time PCR results with similar amplification efficiency among samples. Data represent the quantification of dilutions 0.1 and 10 as the mean ± SEM for 12 experiments performed in triplicate.

From: Model based analysis of real-time PCR data from DNA binding dye protocols

Dilution

Analysis method

 

1

2

3

4

5

6

7

0.1

0.084 ± 0.003

0.11 ± 0.0033

0.57 ± 0.30

0.37 ± 0.19

0.75 ± 0.45

0.71 ± 0.63

0.12 ± 0.022

1

1 ± 0.019

1 ± 0.017

1 ± 0.11

1 ± 0.20

1 ± 0.12

1 ± 0.10

1 ± 0.018

10

14 ± 0.680

10 ± 0.35

242 ± 206

83 ± 52

15 ± 5.5

48 ± 1.0

10 ± 1.1

  1. (1)CT method assuming constant amplification efficiency equal to 1 [12].
  2. (2) CT method with amplification efficiency estimated from a dilution series [14].
  3. (3) Amplification efficiency estimated at two product yield thresholds [15].
  4. (4) Amplification efficiency estimated with LinRegPCR software [13].
  5. (5) Amplification efficiency estimated with the Tichopad et.al. approach [16].
  6. (6) Amplification efficiency estimated from the model proposed by Liu et.al. [17].
  7. (7) Our model based real-time PCR analysis method (MoBPA).
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BMC Bioinformatics

ISSN: 1471-2105

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