Well registered images. First column corresponds to the raw image. Images in the subsequent columns show the difference between reference image and raw image, image after approximate registration and fine registration respectively. In these images red channel corresponds to reference image and green channel corresponds to expression image. Note that as in these images (reference and expression) high intensity (or white) corresponds to low/no expression and vice versa. The last column has the registered image. Rows correspond to genes Cpeb1 (Maximum mutual information = 0.5962), Esr2(0.6118), Rasd2(0.6285) and Tdo2(0.6209) respectively. About 55% of the registrations were of this quality.