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Figure 7 | BMC Bioinformatics

Figure 7

From: Nanopore-based kinetics analysis of individual antibody-channel and antibody-antigen interactions

Figure 7

Top: Biotinylated DNA Hairpin Captured in the Nanopore Detector. Bottom: Biotinylated DNA Hairpin HHM-based CCC Analysis. HMM Signal Profile: The 150 feature vectors obtained from the 50-state HMM-EM/Viterbi implementation in [4-7] are: the 50 dwell percentage in the different blockade levels (from the Viterbi trace-back states), the 50 variances of the emission probability distributions associated with the different states, and the 50 merged transition probabilities from the primary and secondary blockade occupation levels (fits to two-state dominant modulatory blockade signals). The first 50 features, corresponding to the dwell times are, effectively, a de-noised histogram of the blockade samples seen in the "active" window between 20% and 70% of the open channel; current.

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