Figure 8

Top: signal and profile after introduction of an excess of streptavidin – a binding event between streptavidin and the biotinylated hairpin is hypothesized to be the cause of the significant shift in the HMM feature vectors, shown in the Bottom Panel. The signal is only observed after introduction of streptavidin, leading us to hypothesize its possible direct association with a binding event. The HMM Signal Profile: The 150 feature vectors obtained from the 50-state HMM-EM/Viterbi implementation in [4-7] are: the 50 dwell percentage in the different blockade levels (from the Viterbi trace-back states), the 50 variances of the emission probability distributions associated with the different states, and the 50 merged transition probabilities from the primary and secondary blockade occupation levels (fits to two-state dominant modulatory blockade signals).