Table 1 Normalization effects on metabolite concentration data. Effects of normalization on the quantitation of metabolite concentrations. Concentrations are reported in units of μM in columns 2, 3, 6, and 7. The concentration units in columns 4 and 5 are reported as μM/[Creatinine] (μM).
Postulated identity | Study 1 (μM) | Study 2 (μM) | Study 1 Normalized to Creatinine (μM)/[Crea] (μM) | Study 2 Normalized to Creatinine (μM)/[Crea] (μM) | Study 1 Normalized to TMSP/total intensity (μM) | Study 2 Normalized to TMSP/total intensity (μM) |
|---|---|---|---|---|---|---|
2-OG | 3915 ± 2459 | 6261 ± 2524 | 1.24 ± 0.04 | 1.48 ± 0.55 | 62.5 ± 24.8 | 82.3 ± 25.9 |
Acetate | 986 ± 1145 | 440 ± 547 | 0.33 ± 0.36 | 0.19 ± 0.24 | 16.9 ± 20.6 | 11.1 ± 13.7 |
Citrate | 6563 ± 3896 | 6436 ± 2182 | 2.10 ± 0.58 | 1.61 ± 0.50 | 104.6 ± 36.5 | 90.3 ± 24.6 |
Creatine | 329 ± 544 | 550 ± 811 | 0.12 ± 0.20 | 0.27 ± 0.25 | 7.1 ± 12.3 | 15.9 ± 14.8 |
Creatinine | 3029 ± 1161 | 3710 ± 772 | N/A | N/A | 50.9 ± 13.5 | 57.0 ± 3.0 |
Formate | 342 ± 167 | 403 ± 311 | 0.11 ± 0.04 | 0.10 ± 0.06 | 5.7 ± 2.3 | 5.4 ± 3.0 |
Hippurate | 2209 ± 1118 | 3489 ± 1046 | 0.74 ± 0.26 | 0.81 ± 0.23 | 37.2 ± 15.9 | 45.5 ± 12.1 |
Oxalacetate | 766 ± 298 | 514 ± 180 | 0.28 ± 0.12 | 0.15 ± 0.07 | 13.5 ± 5.7 | 8.7 ± 3.6 |
Taurine | 1011 ± 798 | 3187 ± 1611 | 0.35 ± 0.29 | 0.83 ± 0.47 | 19.5 ± 19.3 | 46.9 ± 25.2 |
trans-Aconitate | 1329 ± 528 | 1582 ± 425 | 0.44 ± 0.06 | 0.38 ± 0.11 | 22.3 ± 6.1 | 21.5 ± 60.0 |