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Table 2 The primer sequences for the real-time PCR experiments. The gene specific primer for tobacco (Nicotiana tabacum) NtWBC1 gene and Arabidopsis AtWBC19 gene were designed as follows.

From: Statistical tools for transgene copy number estimation based on real-time PCR

Gene Name

Forward Primer

Reverse Primer

NtWBC1

ATCTCACGTAGCCGGAGCA

TTTGTTCTGGTGGACGGGAT

AtWBC19

AAGAAACGCGGCGAACAC

GCCGTCTCTCTCCTCCAGAA

AtWBC19

TTAGCCAAACGGTACATGAAAAAC

CCAGTCACCATTACCGTAGCAA