Y-aptamer with DNA overhang that binds complement. Top: signal profiles before and after binding. Bottom: the dwell-time distributions on the three dominant levels indicated in the unbound blockade signal. The profiles are surprisingly different, the bound case, with annealed complement, appears to be more "stable", with only two dominant blockade levels. This is consistent with it being a molecule with fewer degrees of freedom (with 6 T overhang now annealed to 6 A complement).