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Figure 5 | BMC Bioinformatics

Figure 5

From: A Bayesian method for calculating real-time quantitative PCR calibration curves using absolute plasmid DNA standards

Figure 5

To build the plasmid absolute standard construct, long oligonucleotides (~80 bp, Table 1) containing multiple primer binding sequences were designed such that their 3' ends overlapped. The two overlapping fragments were then combined into a single DNA molecule using overlap extension PCR [29]. Each partially overlapping fragment generated from the initial overlap extension was combined by a second overlap extension into a single full-length DNA construct.

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