A Bayesian method for calculating real-time quantitative PCR calibration curves using absolute plasmid DNA standards

Table 1 Oligonucleotides and probe used in study.

	Sequence 5' → 3'	Reference
Btheta	F-CGTTCCATTAGGCAGTTGGT	[30]
	R-ACACGGTCCAAACTCCTACG
Entero1	F-AGAAATTCCAAACGAACTTG	[31]
	R-AATGATGGAGGTAGAGCACTGA
Entero2	F-GAGGACCGAACCCACGTA	[32]
	R-CAGTGCTCTACCTCCATCATT
HF183	F-ATCATGAGTTCACATGTCCG	[32, 33]
	R-CCGTCATCCTTCACGCTACT
UC1F1	CCGTCATCCTTCACGCTACTGAGGACCGAACCCACGTACCCTTC	This Study
	AGTGCCGCAGTCGTTCCATTAGGCAGTTGGTGAGAAA
UC1R1	CCTGCCGTCTCGTGCTCCTCAAACGCTTCTTAGTCAGGTACCGT
	CAAGTTCGTTTGGAATTTCTCACCAACTGCCTAATG
UC1F2	TGAGGAGCACGAGACGGCAGGAACCTTCCTCTCAGAACCCCAATG
	ATGGAGGTAGAGCACTGACACGGTCCAAACTCCTA
UC1R2	GATCATGAGTTCACATGTCCGCGTCGCAGGATGTCAAGACAGTA
	AATCCGGATAACGCTCGTAGGAGTTTGGACCGTGTCA
UC1	[TET] CCTGCCGTCTCGTGCTCCTCA [TAMRA]*	[35]

An * indicates that the TaqMan^® probe was modified from the previously reported UT Probe [35].

ISSN: 1471-2105