Performance of annot8r EC-annotation. The plot shows the fraction of correct EC predictions as a function of BLAST similarity scores. Three categories were defined: all four EC levels correct (e.g. EC 22.214.171.124, vertically hatched in figure), the top three EC hierarchy levels correct (e.g. EC 1.2.3.x, diagonally hatched) and incorrect. The total number of annotations for each group of BLAST scores is: score >500: 42329; 300–500: 18692; 200–300: 8024; 150–200: 3033; 100–150: 2173; and 45–100: 1459.