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Table 2 Comparison of TOFI-alpha and TOFI-beta for the F. tularensis genome on 74 processors

From: A high-throughput pipeline for designing microarray-based pathogen diagnostic assays

  

TOFI-alpha

TOFI-beta

Stage

Step

Time

Output

Time

Output

1

MUMmer with near-neighbor

1 m

1730 candidates, 1889863 bp

1 m

1730 candidates, 1889863 bp

1

MUMmer with Proteobacteria

-

-

8 m

1882 candidates, 1768235 bp

1

MUMmer with nt database

-

-

23 m

2140 candidates 1192259 bp

2

OMP

2 m

33299 probes

2 m

18668 probes

3

bl2seq with near-neighbor

-

-

5 m

6979 probes

3

BLAST with Proteobacteria

-

-

1 hr 37 m

3380 probes

3

BLAST with nt database

22 hrs

1469 fingerprints

2 hrs 13 m

1469 fingerprints

 

Total execution time

22 hrs

 

4 hrs 29 m

 
  1. For both TOFI-alpha and TOFI-beta, T = 9 is used as the sole specificity criterion.